Developing Effective Hit Finding Strategies

Discovering and developing a new drug is a complex and time-consuming endeavor. Pharmaceutical and biotechnology companies are under tremendous pressure to be first to market, yet many of the best validated targets are in challenging protein classes where there is little precedence for small molecule ligand binding. This creates a significant hurdle for hit finding. A carefully devised strategy is, therefore, essential when looking for validated hit series with a good chance of being developed into drug-like compounds.

This ebook considers best practice strategies for hit finding.

Which method to use?

  • Knowledge-based approach
  • Target Protein Degradation (TPD)
  • High through-put screening (HTS)
  • Fragment screening for biophysical, target-based approaches
  • Phenotypic, cell functional assays for the discovery of novel targets, mechanisms and drugs

Hit finding trends

  • Cytokine targets
  • Undruggable targets
  • RNA targets and TDP

New technologies

  • Affinity-selection mass spectrometry (ASMS)
  • Native MS
  • AI in hit finding

It is important to develop a strategy that uses the most appropriate hit finding approaches. Depending on the budget, more than one technique could be used to maximize the chances of identifying a strong starting point for the subsequent optimization process. Learn more in this new ebook by Sygnature Discovery.

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