Optimizing qPCR can seem overwhelming and requires careful experimental design that evaluates several performance factors for each reagent formulation tested. Promega has developed guidelines and considerations for successful qPCR optimization by assessing key qPCR performance factors, including specificity, reliability, repeatability, linearity, sensitivity and efficiency. This White Paper:

• Provides an introduction to real-time qPCR and discusses the different approaches to real-time qPCR
• Outlines considerations to help you quickly compare previously optimized qPCR assays with a new qPCR Master Mix
• Offers guidance for optimizing your real-time qPCR assay
• Provides a tool for comparing qPCR reagent formulations